助理教授 陽明大學 醫學生物技檢驗學系(2012.8~2017.7)

博士後研究  Terry Fox Laboratory, BC Cancer Agency, Canada (2011.7~2012.7)

博士後研究  Department of Microbiology and Immunology, University of British Columbia, Canada (2008.11~2011.6)

博士後研究 台灣大學 生化科學所 (2008.2~2008.10)

       自然殺手細胞屬於免疫系統中非專一性防禦系統，負責毒殺受感染細胞和腫瘤細胞。其藉由表面的活化型和抑制型接受器與目標細胞上的ligand結合，此結合可分別誘發活化或抑制性訊號，此兩者訊號的強弱調控其毒殺活性。

探討:

1. 自然殺手細胞對白血病細胞以及癌症細胞的毒殺作用機轉
2. 癌細胞躲避免疫監測系統中自然殺手細胞毒殺作用的機轉
3. 細胞表面醣結構的變化對自然殺手細胞表面接受器的辨識及毒殺功能的影響

1. Lee CY, Suk FM, Twu YC and Liao YJ*. Long-term exposure to low-dose Di-(2-ethylhexyl) phthalate impairs cholesterol metabolism in hepatic stella cells and exacerbates liver librosis. International Journal of Environmental Research and Public Health 17: 3802 
2. Huang HT, Su SC, Chiou TJ, Lin YH, Shih YJ, Wu YX, Fan TH, and Twu TC*.  2020. DNA methylation-mediated Siglec-7 regulation in natural killer cells via two 5’ promoter CpG sites. Immunology 160: 38-51


3. Wang YH, Suk FM, Liu CL, Chen TL, Twu YC*, Hsu MH*, Liao YJ*. 2020. Antifibrotic effects of a barbituric acid derivative on liver fibrosis by blocking the NF-Kb signaling pathway in hepatic stellate cells. Frontiers in Pharmacology 11: 388


4. Wang YH, Liu CL, Chiu WC, Twu YC, Liao YJ*. 2019. HMGCS2 mediates ketone production and regulates the proliferation and metastasis of hepatocellular carcinoma. Cancers 11: 1876


5. Huang CH, Liao YJ, Chiou TJ, Huang HT, Lin YH, Twu YC*. 2019.  TGF-b regulated leukemia cell susceptibility against NK targeting through the down-regulation of the CD48 expression. Immunobiology 224: 649-658


6. Wang YH, Twu YC, Wang CK, Lin FZ, Lee CY, and Liao YJ*. 2018. Niemann-Pick Type C2 protein regulates free cholesterol accumulation and influences hepatic stellate cell proliferation and mitochondrial respiration function.  International Journal of Molecular Sciences 19


7. Yeh CC, Chang CJ, Twu YC, Liu BS, Huang JT, Hung ST, Chu CC, Chan YS, Tsai YJ, Lin SW, Lin M, and Yu LC*. 2018. The molecular genetic background leading to formation of the human erythroid-specific Xg^a/CD99 blood groups. Blood Advances 2: 1854-1864


8. Huang CH, Liao YJ, Fan TH, Chiou TJ, Lin YH, Twu YC*. 2018. A developed NK-92MI cell line with Siglec-7neg phenotype exhibits high and sustainable cytotoxicity against leukemia cells. International Journal of Molecular Sciences 19


9. Yeh CC, Chang CJ, Twu YC, Hung ST, Tsai YJ, Liao JC, Huang JT, Kao YH, Lin SW, Yu LC* 2018. The differential expression of the blood group P¹-A4GALT and P²-A4GALT alleles is stimulated by the transcription factor early growth response 1. Transfusion 58: 1054-1064


10. Lee YH, Liao YJ, Huang CH, Chang FL, Fan TH, and Twu YC*. 2017. Branched I antigens on leukemia cells enhanced sensitivity against NK cytotoxicity through affecting the target-effector interaction. Transfusion 57: 1040-1051
11. Hsu MH, Hsu SM, Kuo YC, Liu CY, Hsieh CY, Twu YC, Wang CK, Wang YH, Liao YJ*. 2017. Treatment with low-dose sorafenib in combination with a novel benzimidazole derivative bearing a pyrolidine side chain provides synergistic anti-proliferative effects against human liver cancer. RSC Advances 7: 16253-16263 


12. Liao YJ, Lee YH, Chang FL, Ho H, Huang CH, and Twu YC*. 2016. The SHP-2-ERK2 signaling pathway regulates branched I antigen formation by controlling the binding of C/EBPa to the IGnTC promoter region during erythroid differentiation. Transfusion 56: 2691-2702
13. Liao YJ, Lee TS, Twu YC, Hsu SM, Yang CP, Wang CK, Liang YC and Chen YM. 2016 Glycine N-methyltransferase deficiency in female mice impairs insulin signaling and promotes glconeogenesis by modulating the PI3K/Akt pathway in the liver. Journal of Biomedical Science 23: 69-
14. Twu YC, Lee TS, Lin YL, Hsu SM, Wang YH, Liao CY, Wang CK, Liang YC and Liao YJ. 2016. Niemann-Pick type C2 protein mediates hepatic stellate cells activation by regulating free cholesterol accumulation. International Journal of Molecular Science 17: 1-12
15. Twu YC*, Teh HS. 2014. The ThPOK transcription factor differentially affects the development and function of self-specific CD8+ T cells and regulatory CD4+ T cells. Immunology 141:431-445
16. Twu YC, Gold MR, Teh HS. 2011. TNFR1 delivers pro-survival signals that are required for limiting TNFR2-dependent activation-induced cell death (AICD) in CD8(+) T cells. European Journal of Immunology 41: 335- 344
17. Twu YC, Hsieh CY, Lin M, Tzeng CH, Sun CF, Yu LC. 2010. Phosphorylation status of transcription factor C/EBPalpha determines cell-surface poly-LacNAc branching (I antigen) formation in erythropoiesis and granulopoiesis. Blood 115: 2491-2499
18. Wang HR, Hsieh CY, Twu YC, Yu LC. 2008. Expression of the human Sd(a) beta-1,4-N- acetylgalactosaminyltransferase II gene is dependent on the promoter methylation status. Glycobiology 18: 104-113
19. Twu YC, Chen CP, Hsieh CY, Tzeng CH, Sun CF, Wang SH, Chang MS, Yu LC. 2007. I branching formation in erythroid differentiation is regulated by transcription factor C/EBPalpha. Blood 110: 4526-4534
20. Twu YC, Hsieh CY, Yu LC. 2006. Expression of the histo-blood group B gene predominates in AB- genotype cells. Transfusion 46: 1988-1996
21. Lin M, Hou MJ, Twu YC, Yu LC. 2005. A novel A allele with 664G>A mutation identified in a family with the Am phenotype. Transfusion 45: 63-69
22. Yu LC, Twu YC, Chou ML, Reid ME, Gray AR, Moulds JM, Chang CY, Lin M. 2003. The molecular genetics of the human I locus and molecular background explain the partial association of the adult i phenotype with congenital cataracts. Blood 101: 2081-2088
23. Twu YC, Chou ML, Yu LC. 2003. The molecular genetics of the mouse I beta-1,6-N- acetylglucosaminyltransferase locus. Biochemical Biophysical Research Communications 303: 868-876
24. Sun CF, Yu LC, Chen IP, Chou DL, Twu YC, Wang WT, Lin M. 2003. Molecular genetic analysis for the Ae1 and A3 alleles. Transfusion 43: 1138-1144
25. Yu LC, Twu YC, Chou ML, Chang CY, Wu CY, Lin M. 2002. Molecular genetic analysis for the B(3) allele. Blood 100: 1490-2
26. Yu LC, Twu YC, Chang CY, Lin M. 2001. Molecular basis of the Kell-null phenotype: a mutation at the splice site of human KEL gene abolishes the expression of Kell blood group antigens. Journal of Biological Chemistry 276: 10247-10252
27. Yu LC, Twu YC, Chang CY, Lin M. 2001. Molecular basis of the adult i phenotype and the gene responsible for the expression of the human blood group I antigen. Blood 98: 3840-3845
28. Yu LC, Chu CC, Chan YS, Chang CY, Twu YC, Lee HL, Lin M. 2001. Polymorphism and distribution of the Secretor alpha(1,2)-fucosyltransferase gene in various Taiwanese populations. Transfusion 41: 1279-1284
29. Yu LC, Chang CY, Twu YC, Lin M. 2000. Human histo-blood group ABO glycosyltransferase genes: different enhancer structures with different transcriptional activities. Biochemical Biophysical Research Communications 273: 459-466
30. Hsu HY, Twu YC. 2000. Tumor necrosis factor-alpha -mediated protein kinases in regulation of scavenger receptor and foam cell formation on macrophage. Journal of Biological Chemistry 275: 41035-41048