跳到主要內容區塊

醫學生物技術暨檢驗學系 (醫技系)

國立陽明大學

黃尉倫

專任助理教授 

黃尉倫     

Wei-Lun Hwang

 

 

聯絡方式 學歷

☏ +886-2-28267000 ext: 5833 (Lab) / 5832 (Office)

 a85296658@ym.edu.tw/a85296658@gmail.com

〒 Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, No. 155, Sec. 2, Linong St., Beitou District, Taipei 112, Taiwan

Sep 2008~Aug 2012, Ph.D, Institute of Biochemistry and Molecular Biology (Taiwan International Graduate Program, TIGP), National Yang Ming University, Taipei, Taiwan


Sep 2006~Jun 2008 M.S., Institute of Microbiology and Immunology, National Yang Ming University, Taipei, Taiwan

 

Sep 2002~Jun 2006 B.S., School of Medical Laboratory Science and Technology, Taipei Medical University, Taipei, Taiwan

 經歷
Aug 2018~present, Assistant Professor, Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University, Taipei, Taiwan
 
2018 Aug~present, Member of Cancer Progression Research Center (tumor microenvironment group), National Yang Ming University, Taipei, Taiwan
 
Oct 2015~Jul 2018, Assistant Professor, The Ph.D Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan

Oct 2012~Jul 2015, Post-Doctoral Fellow, Institute of Microbiology and Immunology and Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
 
研究方向

Research Interests

Cancer Biology, Tumor Immunology, Tumor Microenvironment, Intestinal Biology

Research Direction:

Cancer cells possess characters similar to healthy stem cells, and the degree of cell dedifferentiation is correlated with poor prognosis. Given our rudimentary knowledge toward mechanisms governing stem cell expansion in bulky tumors, we set out to evaluate stem-like cell isolation approaches in colorectal carcinoma (CRC) and identify essential genes regulating CRC stemness. We isolated cancerous stem cell populations as colonospheres (sphere-derived cancer stem cells, SDCSCs) from primary CRC tissues and cell lines for characterization in vitro and in vivo. Microarray analysis and next-generation sequencing of the colonospheres were used to elucidate their transcriptome and miRNome for exploring their enriched biological activities. We found expanded colonospheres fulfilling the cancer stem cell criteria were enriched with CD44(+)/CD166(+) subpopulations and had undergone epithelial-mesenchymal transition (EMT) with Snail being the predominant regulator. The Snail-IL8 signal axis and Snail-miR-146a-Numb circuits were demonstrated to maintain Nanog/OCT4 expression for maintaining tumor niches and Wnt signal cassettes for symmetric stem cell division, respectively. In colorectal cancer patients, Snail and IL8 were coexpressed with the stem cell marker CD44, but not with CD133 or CD24, in CRC tissues and the SnailHighNumbLow profile was correlated with cetuximab resistance and a poorer prognosis. Dr. Hwang’s studies reveal the presence of a complex Snail-induced interactome in CRC-CSCs, proving potential drug targets for advanced CRC patients.

Research Highlights:

  1. Wnt-driven RAB27B activation mediates the CSC-CRC cell interaction through exosomal miR-146a-5p.
  2. CSCs elicit the pattern recognition response in host neutrophils through exosomal non-coding RNAs.
  3. Lymphotoxin-β dictates the Cetuximab resistance in head-and-neck cancer.
  4. The asymmetric-to symmetric (ACD-SCD) division mode of CSC maintains stem-like population.
  5. Snail-IL8 axis introduces cancer stemness in non-CSC cells and sustains the self-renewal of CSCs

 

Teaching Experience

General Biology, Molecular Cell Biology, Cell Biology, Translational Medicine,
Clinical Biochemistry, Bioinformatics Tools

 

研究著作
  1. Ho WT, Chang JS, Chou SF, Hwang WL, Shih PJ, Chang SW, Yang MH, Jou TS, Wang IJ.   “Targeting non-muscle myosin II promotes corneal endothelial migration through   regulating lamellipodial dynamics.” J Mol Med (Berl). 2019 Sep;97(9):1345-1357.
  2. Cheng WC, Liao TT, Lin CC, Yuan LE, Lan HY, Lin HH, Teng HW, Chang HC, Lin CH, Yang   CY, Huang SC, Jiang JK, Yang SH, Yang MH, Hwang WL. “RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer.” Int J Cancer. 2019 Apr 13.
  3. Hwang WL, Lan HY, Cheng WC, Huang SC, Yang MH. “Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer.” J Hematol Oncol. 2019 Jan 25;12(1):10.
  4. Lee CC, Lin JC, Hwang WL, Kuo YJ, Chen HK, Tai SK, Lin CC, Yang MH. “Macrophage-secreted interleukin-35 regulates cancer cell plasticity to facilitate metastatic colonization.” Nat Commun. 2018 Sep 14;9(1):3763.
  5. Wu MH, Luo JD, Wang WC, Chang TH, Hwang WL, Lee KH, Liu SY, Yang JW, Chiou CT, Chang CH, Chiang WF. “Risk analysis of malignant potential of oral verrucous hyperplasia: A follow-up study of 269 patients and copy number variation analysis” Head Neck. 2018 May;40(5):1046-1056. 
  6. Liao KH, Chang SJ, Chang HC, Chien CL, Huang TS, Feng TC, Lin WW, Shih CC, Yang MH, Yang SH, Lin CH, Hwang WL*, Lee OK*. “Endothelial angiogenesis is directed by RUNX1T1-regulated VEGFA, BMP4 and TGF-β2 expression.” PLoS One. 2017 Jun 22;12(6):e0179758.
  7. Hsu DS*, Hwang WL*, Yuh CH, Chu CH, Ho YH, Chen PB, Lin HS, Lin HK, Wu SP, Lin CY, Hsu WH, Lan HY, Wang HJ, Tai SK, Hung MC, Yang MH. “Lymphotoxin-β Interacts with Methylated EGFR to Mediate Acquired Resistance to Cetuximab in Head and Neck Cancer.” Clin Cancer Res. 2017 Aug 1;23(15):4388-4401.
  8. Hwang WL, Yang MH “Numb is involved in the non-random segregation of subcellular vesicles in colorectal cancer stem cells.” Cell Cycle. 2016 Oct 17;15(20):2697-703 
  9. Liao TT, Hsu WH, Ho CH, Hwang WL, Lan HY, Lo T, Chang CC, Tai SK, Yang M “Let-7 Modulates Chromatin Configuration and Target Gene Repression through Regulation of the ARID3B Complex.” Cell Rep. 2016 Jan 26;14(3):520-33. 
  10. Hwang WL, Jiang JK, Yang SH, Huang TS, Lan HY, Teng HW, Yang CY, Tsai YP, Lin CH, Wang HW, Yang MH.“MicroRNA-146a Directs the Symmetric Division of Snail-Dominant Colorectal Cancer Stem Cells”, Nat Cell Biol. 2014 Mar;16(3):268-80 
  11. Wang HW, Yang SH, Huang GD, Lin JK, Chen WS, Jiang JK, Lan YT, Lin CC, Hwang WL, Tzeng CH, Li AF, Yen CC, Teng HW “Temsirolimus enhances the efficacy of cetuximab in colon cancer through a CIP2A-dependent mechanism”, J Cancer Res Clin Oncol. 2014 Apr;140(4):561-71 
  12. Hwang WL, Yang MH, Tsai ML, Lan HY, Su SH, Chang SC, Teng HW, Yang SH, Lan YT, Chiou SH, Wang HW. "SNAIL Regulates Interleukin-8 Expression, Stem Cell-Like Activity, and Tumorigenicity of Human Colorectal Carcinoma Cells", Gastroenterology. 2011 Jul;141(1):279-91, 291.e1-5 
     
     
榮譽與獎項

2014: Award of Distinguished Thesis for Post-Doctoral Fellow, Ministry of Science and Technology.

2013: Award of Distinguished Thesis, 8th Tien-Te Lee Biomedical Foundation, Taiwan

2012: Award of the Best Research Project, 17th Taiwan Joint Cancer Conference

2012: Excellent Oral Presentation Award, 9th Symposium on Frontiers of Biomedical

     Science and 11th Cross-Strait Symposium on Biomedical Research supported by

     Jung-Yaw Lin Science and Education Foundation.

2012: Silver Award, Merck Millipore Asia Bioforum (Taipei)

 
Lab Members

Research Assistant: 黃詩晴

Master student: 汪雅珮 (MS2),王薏荃 (MS1), 陳立昀 (MS1), 林巧雯 (MS1)

Undergraduate Student: 陳牧澤 (BS3))

 

Previous Lab members

Postdoctoral Researcher: 李志展

Research Assistant: 馮德嘉

Undergraduate Student: 練冠霆 (北醫大營養保健系), 林煒樺 (北醫大藥學系), 羅正廷 (北醫大醫學系); 3位同學均獲得科技部大專生專題研究計畫補助

 

Lab Activities

2018實驗室聚餐

 

2019實驗室聚餐