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醫學生物技術暨檢驗學系 (醫技系)




Wailap Victor Ng



聯絡方式   學歷


Persional web site


Ph.D., University of Massachusetts


  1. Systems biology - Apply DNA microarray, mass spectrometry, 2-hybrid expression system, and bioinformatics technologies to (i) characterize the biomodules essential for cell survival upon environmental perturbations in Halobacterium sp. NRC-1 and Haloarcula marismortui and (ii) identify the new functions of more than 50% of all the predicted genes in these microorganisms.
  2. Biomarker discovery - Identify cancer diagnostic markers and therapeutic targets using bioinformatics and mass spectrometry tools.
  3. Technology development - EST database mining, mass spectrometry, and mass spectrometry data analysis system.

  1. Timothy H Wu, Lichieh J Chu, Jian-Chiao Wang, Ting-Wen Chen, Yin-Jing Tien, Wen-Chang Lin and Wailap V Ng*. Meta-analytical biomarker search of EST expression data reveals three differentially expressed candidates. BMC Genomics (In press).
  2. Liu YC, Lin IH, Chung WJ, Hu WS, Ng WV, Lu CY, Huang TY, Shu HW, Hsiao KJ, Tsai SF, Chang CH, Lin CH. Proteomics characterization of cytoplasmic and lipid-associated membrane proteins of human pathogen Mycoplasma fermentans M64. PLoS One. 7(4):e35304, 2012.
  3.  Shu H-W, Liu T-T, Chang H-Y, Liu Y-M, Wu K-M, Shu H-Y, Tsai S-F, Hsiao K-J, S. Hu WS*, and Ng WV*. Complexity of the Mycoplasma fermentans M64 genome and metabolic essentiality and diversity among mycoplasmas. PLoS One. 7(4):e32940, 2012.
  4. Chen TW, Wu TH, Ng WV, Lin WC. Interrogation of alternative splicing events in duplicated genes during evolution. BMC Genomics. 12 Suppl 3:S16, 2011.
  5. Shu H-W, Liu T-T, Chang H-Y, Liu Y-M, Wu K-M, Shu H-Y, Tsai S-F, Hsiao K-J, S. Hu WS*, and Ng WV*. Genome sequence of the repetitive sequence rich Mycoplasma fermentans strain M64. J Bacteriol 193(16):4302-3, 2011.
  6. Chu LJ, Yang H, Shih P, Kao Y, Tsai YS, Chen J, Huang G, Weng RR, Ting YS, Fang X, von Haller PD, Goodlett DR, Ng WV*. Metabolic capabilities and systems fluctuations in Haloarcula marismortui revealed by integrative genomics and proteomics analyses. J Proteome Res. 10(7):3261-73, 2011.
  7. Liao WC, Ng WV, Lin IH, Syu WJ, Liu TT, Chang CH. T4-like Genome Organization of the Escherichia coli O157:H7 Lytic Phage AR1. J Virol. 85(13):6567-78, 2011.
  8. Ting YS, Shaffer SA, Jones JW, Ng WV, Ernst RK, Goodlett DR. 2011. Automated lipid a structure assignment from hierarchical tandem mass spectrometry data. J Am Soc Mass Spectrom. 22(5):856-66.
  9. Chu LJ, Chen MC, Setter J, Tsai YS, Yang H, Fang X, Ting YS, Shaffer SA, Taylor GK, von Haller PD, Goodlett DR, Ng WV*. 2011. New structural proteins of Halobacterium salinarum gas vesicle revealed by comparative proteomics analysis. J Proteome Res. 10(3):1170-8.
  10. Song YC, Tang SJ, Lee TP, Hung WC, Lin SC, Tsai CY, Ng WV, Wu MF, Sun KH. 2010. Reversing interleukin-2 inhibition mediated by anti-double-stranded DNA autoantibody ameliorates glomerulonephritis in MRL-lpr/lpr mice. Arthritis Rheum. 62(8):2401-11.
  11. Ho, M.Y., Tang, S.J., Ng, W.V., Yang, W., Leu, S.J., Lin, Y.C., Feng, C.K., Sung, JS, Sun, K.H. 2010. Nucleotide-binding domain of phosphoglycerate kinase 1 reduces tumor growth by suppressing COX-2 expression. Cancer Sci. 101(11):2411-6.
  12. Rajkumar, R., Ilayaraja, R., Liao, C.C., Archunan, G., Achiraman, S., Prakash, S., Ng, W.V., Tsay, Y.G. 2010. Detection of alpha (2u)-globulin and its bound putative pheromones in the preputial gland of the Indian commensal rat (Rattus rattus) using mass spectrometry. Rapid Commun Mass Spectrom. 24(6):721-8.
  13. Rajkumar, R., Karthikeyan, K., Archunan, G., Huang, P.H., Chen, Y.W., Ng, W.V., Liao, C.C. 2010. Using mass spectrometry to detect buffalo salivary odorant-binding protein and its post-translational modifications. Rapid Commun Mass Spectrom.  24(22):3248-54.
  14. Fu, H.Y., Lin, Y.C., Chang, Y.N., Tseng, H., Huang, C.C., Liu, K.C., Huang, C.S., Su, C.W., Weng, R.R., Lee, Y.Y., Ng, W.V., Yang, C.S. 2010. A novel six-rhodopsin system in a single archaeon. J Bacteriol. 192(22):5866-73.
  15. Chen, T.W., Wu, T.H., Ng, W.V., Lin, W.C. 2010. DODO: an efficient orthologous genes assignment tool based on domain architectures. Domain based ortholog detection. BMC Bioinformatics. 15;11 Suppl 7:S6.
  16. Wu, T.H., Chang, I.Y., Chu, L.C., Huang, H.C.*, Ng, W.V.*. 2010. Modularity of Escherichia coli sRNA regulation revealed by sRNA-target and protein network analysis. BMC Bioinformatics. 11 Suppl 7:S11
  17. Ng, W.V., Berquist, B.R., Coker, J.A., Capes, M., Wu, T.H., DasSarma, P., DasSarma, S. 2008. Genome sequences of Halobacterium species. Genomics. 2008 Jun;91(6):548-52
  18. Gan, R.R., Yi, E.C., Chiu, Y., Lee, H., Kao, Y.C., Wu, T.H., Aebersold, R., Goodlett, D.R., Ng, W.V.*. 2006. Proteome analysis of Halobacterium sp. NRC-1 facilitated by the biomodule analysis tool BMSorter.. Mol Cell Proteomics. 5(6):987-97.
  19. Ng, W.V., Berquist, B.R., Coker, J.A., Capes, M., Wu, T.H., DasSarma, P., DasSarma, S. 2008. Genome sequences of Halobacterium species. Genomics 91:548-52
  20. Gan, R.R., Yi, E.C., Chiu, Y., Lee, H., Kao, Y.C., Wu, T.H., Aebersold, R., Goodlett, D.R., and Ng, W.V. * 2006. Proteome analysis of Halobacterium sp. NRC-1 facilitated by the biomodules analysis tool BMSorter. Mol Cell Proteomics 5:987-97.
  21. Zeng, F., Hon, C.C., Sit, W.H., Chow, K.Y., Hui, R.K., Law, I.K., Ng, V.W., Yang, X.T., Leung, F.C., and Wan, JM. 2005. Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray. Int J Oncol. 27:513-23.
  22.  Baliga, N.B., Bonneau, R., Facciotti, M.T., Pan, M., Glusman, G., Deutsch, E.W., Shannon, P., Chiu, Y., Weng, R.S., Gan, R.R., Hung, P., Date, S., Marcotte, E., Hood, L., and Ng, W.V.* 2004. Genome of Haloarcula marismortui – a halophilic archaeon from the Dead Sea. Genome Research 14:2221-34.
  23. Goo, Y.A., Roach, J., Glusman, G., Baliga, N.S., Deutsch, K., Pan, M., Kennedy, S., DasSarma, S, Ng W.V., and Hood L. 2004. Low-pass sequencing for microbial comparative genomics. BMC Genomics 5:3-21.
  24. Goo, Y.A., Yi, E.C., Baliga, N.S., Tao, W.A., Pan, M., Aebersold, R., Goodlett, D.R., Hood, L., and Ng, W.V.* 2003. Proteomic analysis of an extreme halophilic archaeon, Halobacterium sp. NRC-1. Mol Cell Proteomics 2:506-24.
  25. Baliga, N.S., Pan, M., Goo, Y.A., Yi, E.C., Goodlett, D.R., Dimitrov, K., Shannon, P., Aebersold, R., and Ng, W.V. , Hood, L. 2002. Coordinate regulation of energy transduction modules in Halobacterium sp. analyzed by a global systems approach. Proc Natl Acad Sci U S A 99:14913-8.
  26. Kennedy, S.P., Ng, W.V., Salzberg, SL., Hood, L., and DasSarma, S. 2001. Understanding the adaptation of Halobacterium species NRC-1 to its extreme environment through computational analysis of its genome sequence. Genome Research 11:1641-50.
  27. Baliga, N.S., Kennedy, S.P., Ng, W.V., Hood, L., and DasSarma, S. 2001. Genomic and genetic dissection of an archaeal regulon. Proc Natl Acad Sci U S A 98:2521-5.
  28. Ø Peck, R.F., Echavarri-Erasun, C., Johnson, E.A., Ng, W.V., Kennedy, S.P., Hood, L., DasSarma S., and Krebs, M.P. 2001. brp and blh are required for synthesis of the retinal cofactor of bacteriorhodopsin in Halobacterium salinarum. J. Biol. Chem 276:5739-44.
  29. DasSarma, S., Kennedy, S.P., Berquist, B., Ng, W.V., Baliga, N.S., Spudich, J.L., Krebs, M.P., Eisen, J.A., Johnson, C.H., and Hood, L. 2001. Genomic perspective on the photobiology of Halobacterium species NRC-1, a phototrophic, phototactic, and UV-tolerant haloarchaeon. Photosyn. Res. 70:3-17.
  30. Ng, W.V., S. P. Kennedy, G. G. Mahairas, B. Berquist, M. Pan, H. D. Shukla, S. R. Lasky, N. S. Baliga, V. Thorsson, J. Sbrogna, S. Swartzell, D.Weir, J. Hall, T. A. Dahl, R.Welti, Y. A. Goo, B. Leithauser, K. Keller, R. Cruz, M. J. Danson, D.W. Hough, D. G. Maddocks, P. E. Jablonski, M. P. Krebs, C. M. Angevine, H. Dale, T. A. Isenbarger, R. F. Peck, M. Pohlschroder, J. L. Spudich, K. W. Jung, M. Alam, T. Freitas, S. Hou, C. J. Daniels, P. P. Dennis, A. D. Omer, H. Ebhardt, T. M. Lowe, P. Liang, M. Riley, L. Hood, and S. DasSarma. 2000. Genome sequence of Halobacterium sp. NRC-1. Proc Natl Acad Sci 97:12176-81.
  31. Baliga, N.S., Goo, Y.A., Ng, W.V., Hood, L., Daniels, C.J., and DasSarma, S. 2000. Is gene expression in Halobacterium NRC-1 regulated by multiple TBP and TFB transcription factors? Mol. Microbiol. 36:1184-5.
  32. Nelson, P.S., Hawkins, V., Schummer, M., Bumgarner, R, Ng, W.-L., Ideker, T., Ferguson, C., and Hood, L. 1999 Negative selection: a method for obtaining low-abundance cDNAs using high-density cDNA clone arrays. Genet Anal 15:209-15.
  33. Schummer, M., Ng, W.V., Bumgarner, R.E., Nelson, P.S., Schummer, B., Bednarski, D.W., Hassell, L., Baldwin, R.L., Karlan, B. Y., and Hood, L. 1999. Comparative hybridization of an array of 21,500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas. Gene 238:375-85.
  34. Huang, G.M., Ng, W.-L., Farkas, J., He, L., Liang, H.A., Gordon, D., Yu, J., and Hood, L. 1999. Prostate cancer expression profiling by cDNA sequencing analysis. Genomics 59:178-86.
  35. Wang, K, Gan, L., Jeffery, E., Gayle, M., Gown, A.M., Skelly, M., Nelson, P.S., Ng, W.V., Schummer, M., Hood, L., and Mulligan, J. 1999. Monitoring gene expression profile changes in ovarian carcinomas using cDNA microarray. Gene 229:101-8.
  36. Nelson, P.S., Ng, W.-L., Schummer, M., True, L.D., Liu, A.L., Bumgarner, R., Ferguson, C., Dimak, A., and Hood, L. 1998. An expressed-sequence-tag database of the human prostate: Sequence analysis of 1,168 cDNA clones. Genomics 47: 12-25.
  37. Ng, W.-L. et al. 1998. Snapshot of a large dynamic replicon in a halophilic archaeon: megaplasmid or minichromosome? Genome Research 8:1131-41.
  38. M. Schummer, Ng, W.-L., Nelson, P. S., Bumgarner, R.E, and Hood, L. 1997. An inexpensive and-held device for the construction of high density nucleic acid arrays. Biotechniques 23: 1087-1092.
  39. Ng, W.-L.*, Schummer, M., Cirisano, F.D., Baldwin, R.E., Karlan, B.Y., and Hood, L. 1996. High-throughput plasmid mini preparation facilitated by micro-mixing. Nucleic Acids Res. 24:5045-5047.
  40. Ng, W.-L., Arora, P., and DasSarma, S. 1994. Large deletion in class III gas vesicle-deficient mutants of Halobacterium halobium. System. Appl. Microbiol. 16: 560-568.
  41. Bobovnikova, Y., Ng, W.-L., DasSarma, S., and Hackett, N.R. 1994. Restriction mapping the genome of Halobacterium halobium strain NRC-1. System. Appl. Microbiol. 16: 597-604.
  42. Ng, W.-L. and DasSarma, S. 1993. Minimal replication origin of the 200 kilobase Halobacterium plasmid pNRC100. J. Bacteriol. 175: 4584-4596.
  43. Halladay, J.T, Ng, W.-L., and DasSarma, S. 1992. Genetic transformation of a halophilic archaebacterium with a gas vesicle gene cluster restores its ability to float. Gene 119: 131-136.
  44. Ng, W.-L., Kothakota, S., and DasSarma, S. 1991. Structure of the gas vesicle plasmid in Halobacterium halobium: inversion isomers, inverted repeats, and insertion sequences. J. Bacteriol. 173:1958-1964.
  45. J.G. Jones, Hackett, N.R., Halladay, J.T, Scothorn, D.J., Yang, C.-F., Ng, W.-L., and DasSarma, S. 1989. Analysis of insertion mutants reveals two new genes in the pNRC100 gas vesicle cluster of Halobacterium halobium. Nucleic Acids Res. 17:7785-7793.
  46. Ng, W.-L. and DasSarma ,S. 1991. Physical and genetic mapping of the unstable gas vesicle plasmid in Halobacterium halobium NRC-1. In F. Rodriguez-Valera (ed.), “General and applied aspects of halophilic microorganisms”, Plenum Press, New York, pp 305-311. (non SCI)